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The Experimental Medicine Division Flow Cytometry Facility based in Room 5605, Level 5, John Radcliffe Hospital offers a high-quality Flow Cytometry service to a variety of users, using a wide variety of techniques. The Facility was initially funded with a combination of funds from the Translational Gastroenterology Unit, Biomedical Research Council and Nuffield Department of Clinical Medicine.

The Facility comprises BD SORP LSRII & BD LSRFortessa analysers, a Cytek Aurora spectral cytometer, plus a BD FACSAriaIII cell sorter. All instruments in the Facility are on maintenance contracts so that they are regularly serviced and can be repaired should any problems arise. We offer training for the instruments, assistance planning your experiments and can advise regarding your data analysis; we can supply you with access to the analysis software FlowJo, Tree-Star, Inc (registration fee applicable) as part of our site license. We are also happy to give an introduction to flow cytometry for those new to the technique.

If you are interested in gaining access to the Facility or would like to know more, please contact the Facility Manager Helen Ferry (01865 228964). We strive to make the Facility as good as possible, so please contact the Facility Manager if you have any suggestions or constructive feedback (positive or negative) regarding any aspect of the Facility, so that we can continue to improve and offer the best possible service to our users.

It is advised that users follow the ‘Minimum Information about a Flow Cytometry Experiment’ recommendations when planning/performing an experiment. These guidelines were proposed by the International Society for Advancement of Cytometry (ISAC) in 2008 to raise the standard of published flow cytometry data. The recommendations can be downloaded from the ISAC website.

Helen Ferry is member of the Royal Microscopical Society, Flow Cytometry Section Committee

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(Images used courtesy of BD Biosciences)