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Snakebite envenoming is a serious problem in Myanmar. The great majority of snakebite in this country is due to Russell's Viper (Daboia siamensis). For many years, the Burma Pharmaceutical Industry has produced a monovalent antivenom to Russell's Viper in horses. At present, the only way of determining the level of antibody against D. siamensis venom in hyperimmune horse serum is to perform venom neutralisation tests in mice. In this study, we describe the development of an in vitro ELISA assay to estimate neutralising capacity of horse serum. We found a strong correlation between the ELISA assay and the venom neutralisation test in mice (r = 0.982). The assay is robust and has sufficient sensitivity (92%) and specificity (96%) to replace the venom neutralisation test in mice during the immunisation phase in horses.

Original publication

DOI

10.1016/j.toxicon.2018.07.012

Type

Journal article

Journal

Toxicon

Publication Date

01/09/2018

Volume

151

Pages

163 - 168

Keywords

Antivenom, Daboia siamensis, ELISA, Myanmar, Russell's Viper, Snakebite, Animals, Antibodies, Antivenins, Enzyme-Linked Immunosorbent Assay, Horses, Immunization, Russell's Viper, Viper Venoms