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Chronic obstructive pulmonary disease (COPD) is associated with pulmonary inflammation with increased numbers of macrophages located in the parenchyma. These macrophages have the capacity to mediate the underlying pathophysiology of COPD; therefore, a better understanding of their function in chronic inflammation associated with this disease is vital. Ion channels regulate many cellular functions; however, their role in macrophages is unclear. This study examined the expression and function of transient receptor potential (TRP) channels in human macrophages. Human alveolar macrophages and lung tissue macrophages expressed increased mRNA and protein for TRPC6 when compared with monocytes and monocyte-derived macrophages. Moreover, TRPC6 mRNA expression was significantly elevated in alveolar macrophages from patients with COPD compared with control subjects. There were no differences in mRNA for TRPC3 or TRPC7. Although mRNA for TRPM2 and TRPV1 was detected in these cells, protein expression could not be determined. Fractionation of lung-derived macrophages demonstrated that TRPC6 protein was more highly expressed by smaller macrophages compared with larger macrophages. Using whole-cell patch clamp electrophysiology, TRPC6-like currents were measured in both macrophage subpopulations with appropriate biophysical and basic pharmacological profiles. These currents were active under basal conditions in the small macrophages. These data suggest that TRPC6-like channels are functional on human lung macrophages, and may be associated with COPD.

Original publication

DOI

10.1165/rcmb.2008-0373OC

Type

Journal article

Journal

Am J Respir Cell Mol Biol

Publication Date

09/2010

Volume

43

Pages

296 - 304

Keywords

Adult, Blotting, Western, Case-Control Studies, Electrophysiology, Female, Flow Cytometry, Humans, Macrophages, Alveolar, Male, Microscopy, Confocal, Middle Aged, Monocytes, Patch-Clamp Techniques, Pulmonary Disease, Chronic Obstructive, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, TRPC Cation Channels, TRPC6 Cation Channel, TRPM Cation Channels, TRPV Cation Channels