Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Barrett's esophagus is an example of a pre-invasive state, for which current endoscopic surveillance methods to detect dysplasia are time consuming and inadequate. The prognosis of cancer arising in Barrett's esophagus is improved by early detection at the stage of mucosal carcinoma or high-grade dysplasia. Molecular imaging methods could revolutionize the detection of dysplasia, provided they permit a wide field of view and highlight abnormalities in real time. We show here that cell-surface glycans are altered in the progression from Barrett's esophagus to adenocarcinoma and lead to specific changes in lectin binding patterns. We chose wheat germ agglutinin as a candidate lectin with clinical potential. The binding of wheat germ agglutinin to human tissue was determined to be specific, and we validated this specific binding by successful endoscopic visualization of high-grade dysplastic lesions, which were not detectable by conventional endoscopy, with a high signal-to-background ratio of over 5.

Original publication

DOI

10.1038/nm.2616

Type

Journal article

Journal

Nat Med

Publication Date

15/01/2012

Volume

18

Pages

315 - 321

Keywords

Aged, Aged, 80 and over, Barrett Esophagus, Esophageal Neoplasms, Esophagoscopy, Esophagus, Female, Fluorescence, Glycosphingolipids, Humans, Male, Middle Aged, Molecular Imaging, Precancerous Conditions, Wheat Germ Agglutinins