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Nontypeable Haemophilus influenzae often causes exacerbations of chronic obstructive pulmonary disease (COPD), and these exacerbations are frequently treated with oral antibiotics. The goals of this study were to determine the frequency of the simultaneous presence of multiple strains of H. influenzae in sputum and to measure the MICs of antibiotics for the isolates. In a prospective study, adults with COPD were seen monthly. Sputum cultures were obtained, and individual colonies were subjected to genomic DNA typing and MIC determinations. Multiple strains of H. influenzae were present simultaneously in the sputum of 26.3% of adults with COPD. In 64.5% of these, MICs of >/=1 antibiotic varied by >/=4-fold among the strains. Therefore, multiple strains of H. influenzae are frequently present simultaneously in the sputum of adults with COPD, and the antimicrobial susceptibility of different strains in the same sputum sometimes differs.

Original publication




Journal article


J Infect Dis

Publication Date





404 - 409


Aged, Aged, 80 and over, Anti-Bacterial Agents, Bacterial Typing Techniques, Colony Count, Microbial, DNA, Bacterial, Female, Haemophilus influenzae, Humans, Lung Diseases, Obstructive, Male, Microbial Sensitivity Tests, Middle Aged, Prospective Studies, Sputum