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Lymphocytic choriomeningitis virus (LCMV), strain WE, is a non-cytopathic RNA virus that is highly adapted to its natural host, the mouse. Acute infection of adult mice leads to generalized virus spread, followed by cytotoxic T lymphocyte-mediated virus clearance below the detection levels of conventional assays within 2-3 weeks. Indirect evidence had suggested that virus or viral antigen might persist in the immune mouse. Here we demonstrate LCMV-WE persistence at low levels after infection with 10(2) or 10(6) plaque-forming units, shown as viral genome, viral antigen, and replicative virus using sensitive in vitro and in vivo assays. The finding that LCMV-WE persists in the face of apparently intact immune responses resembles the situation in some viral (hepatitis B and C, HIV) and bacterial (tuberculosis, leprosy) infections in humans; the results are relevant to the understanding not only of other murine and human persistent viral infections but also of protective immunological memory by "infection immunity."

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

12/10/1999

Volume

96

Pages

11964 - 11969

Keywords

Animals, Antigens, Viral, Base Sequence, Immunohistochemistry, Immunologic Memory, Kidney, Lung, Lymphocytic choriomeningitis virus, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Models, Genetic, Molecular Sequence Data, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Spleen, Time Factors, Tissue Distribution