Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

A method is described for the isolation of parietal cells from the gastric mucosa of the guinea pig by enzymatic digestion with collagenase. A suspension was obtained that contained 70-80% parietal cells. About 80% of the cells were viable immediately after incubation, but viability dropped sharply after one hour. Parietal cells were identified by their morphology on light and electron microscopy, by their uptake of neutral red, by immunofluorescent staining and by carbonic anhydrase activity. Antibodies to four distinct parietal-cell antigens were obtained from rabbits immunized with the isolated parietal cells or fractions thereof. These antibodies were directed against the microsomal fraction of the parietal-cell cytoplasm, the plasma and nuclear membranes, the soluble proteins, and Castle's intrinsic factor. The antibody against the microsomal fraction, though reacting in the same way as the antibody to parietal cell canaliculi found in the serum of patients with pernicious anaemia, showed greater species specificity.


Journal article



Publication Date





603 - 612


Anemia, Pernicious, Animals, Antibodies, Antigen-Antibody Reactions, Antigens, Anura, Cell Membrane, Cell Separation, Cell Survival, Chronic Disease, Cytotoxicity Tests, Immunologic, Fluorescent Antibody Technique, Gastric Mucosa, Gastritis, Guinea Pigs, Humans, Immunization, Intrinsic Factor, Mice, Microscopy, Electron, Microsomes, Rabbits, Rana catesbeiana, Rats, Staining and Labeling, Time Factors