Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3' ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.

Original publication

DOI

10.1038/sj.gene.6363669

Type

Journal article

Journal

Genes Immun

Publication Date

06/2000

Volume

1

Pages

321 - 324

Keywords

Alleles, Base Sequence, DNA Primers, England, Female, Gene Frequency, Haplotypes, Humans, Interleukin-10, Male, Poland, Polymerase Chain Reaction, Polymorphism, Genetic, Promoter Regions, Genetic