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A polymerase chain reaction with sequence-specific primers (PCR-SSP) system using primers with mismatches at the 3' ends was developed to determine polymorphisms in IL-10 promoter region. Three previously described biallelic polymorphisms in IL-10 were linked in a 12 reaction PCR-SSP system and the method used to provide genotype data on 233 UK and 166 Polish controls. There are eight possible polymorphic combinations in IL-10 promoter gene but only three were observed in both control groups. Population frequencies of IL-10 genotypes show, in contrast to HLA, that UK and Polish frequencies are remarkably similar.

Original publication




Journal article


Genes Immun

Publication Date





321 - 324


Alleles, Base Sequence, DNA Primers, England, Female, Gene Frequency, Haplotypes, Humans, Interleukin-10, Male, Poland, Polymerase Chain Reaction, Polymorphism, Genetic, Promoter Regions, Genetic