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An evolutionary analysis was undertaken of HIV-1 gag p17 sequences taken from a small cohort of hemophilia B patients infected from a common batch of clotting factor concentrate. The sequence population found at seroconversion was highly homogeneous, suggesting that the infecting batch also contained little sequence variation. Genetic diversification was found in follow-up sequences taken approximately 3 years later and was generally found to be complex. Greater rates of synonymous to nonsynonymous substitution were observed, especially when comparing distantly related isolates, and the rate of synonymous substitution was used to estimate times of divergence for a number of isolates of HIV-1 including the origin of the subtypes A to H. The p17 region is therefore proposed as a useful marker for future epidemiological studies.

Original publication

DOI

10.1089/aid.1995.11.1197

Type

Journal article

Journal

AIDS Res Hum Retroviruses

Publication Date

10/1995

Volume

11

Pages

1197 - 1201

Keywords

Amino Acid Sequence, Base Sequence, Biological Evolution, Cohort Studies, Follow-Up Studies, Gene Products, gag, Genetic Variation, HIV Antigens, HIV Infections, HIV-1, Hemophilia B, Humans, Molecular Sequence Data, Phylogeny, Sequence Homology, Amino Acid, Transfusion Reaction, Viral Proteins, gag Gene Products, Human Immunodeficiency Virus