I began my research career as an undergraduate at the University of Washington (Seattle, US). While at university, I studied the determinants driving the sensitivity of human immunodeficiency virus to neutralizing antibodies in the labs of Nancy Haigwood and Julie Overbaugh. This initial exposure to research sparked my interest in immunology.
Upon graduation, I moved to Harvard University to pursue a Ph.D. in Virology. I performed my thesis research in the laboratory of Dan Barouch and studied the role of CD4 T cells in regulating adenovirus-vector elicited immune responses. During my thesis research I became interested in understanding the processes regulating CD8 T cell functionality.
To continue my research on CD8 T cell functionality and their involvement in autoimmune diseases, I joined Paul Klenerman’s group to investigate the functionality and clonality of unconventional CD161+ CD8 T cells within human mucosal tissues. Studying these cell populations will give the opportunity to expand my knowledge of T cell regulatory processes and allow me to learn about intestinal autoimmune diseases, a newfound interest of mine.
I currently hold an Oxford-UCB Prize Postdoctoral Fellowship to carry out my research. I am also a member of the American Association of Immunologists.
T cell biology, Intestinal immunity, Inflammatory bowel disease, Flow cytometry, Transcriptional profiling
Role of human intestinal CD161+ CD8+ T cells in inflammatory bowel disease
Provine, N. M.,A. Badamchi-Zadeh, C. A. Bricault, P. Penaloza-MacMaster, R. A. Larocca, E. N. Borducchi, M. S. Seaman, and D. H. Barouch. 2016. Transient CD4+ T cell depletion results in the delayed development of a functional vaccine-elicited antibody response. J Virol 90: 4278-4288.
Penaloza-Macmaster, P., N. M. Provine, E. Blass, and D. H. Barouch. 2015. CD4 T Cell Depletion Substantially Augments the Rescue Potential of PD-L1 Blockade for Deeply Exhausted CD8 T Cells. J Immunol 195: 1054–1063.
Penaloza-MacMaster, P., D. L. Barber, E. J. Wherry, N. M. Provine, J. E. Teigler, L. Parenteau, S. Blackmore, E. N. Borducchi, R. A. Larocca, K. B. Yates, H. Shen, W. N. Haining, R. Sommerstein, L. M. D'Cruz, R. Ahmed, and D. H. Barouch. 2015. Vaccine-elicited CD4 T cells induce immunopathology after chronic LCMV infection. Science 347: 278–282.
Teigler, J. E., P. Penaloza-MacMaster, R. Obeng, N. M. Provine, R. A. Larocca, E. N. Borducchi, and D. H. Barouch. 2014. Hexon Hypervariable Region-Modified Adenovirus Type 5 (Ad5) Vectors Display Reduced Hepatotoxicity but Induce T Lymphocyte Phenotypes Similar to Ad5 Vectors. Clinical and Vaccine Immunology 21: 1137–1144.
Provine, N. M., R. A. Larocca, P. Penaloza-Macmaster, E. N. Borducchi, A. McNally, L. R. Parenteau, D. R. Kaufman, and D. H. Barouch. 2014. Longitudinal Requirement for CD4+ T Cell Help for Adenovirus Vector-Elicited CD8+ T Cell Responses. J Immunol 192: 5214–5225.
Penaloza-MacMaster, P., J. E. Teigler, R. C. Obeng, Z. H. Kang, N. M. Provine, L. Parenteau, S. Blackmore, J. Ra, E. N. Borducchi, and D. H. Barouch. 2014. Augmented Replicative Capacity of the Boosting Antigen Improves the Protective Efficacy of Heterologous Prime-Boost Vaccine Regimens. J Virol 88: 6243–6254.
Im, E. J., E. N. Borducchi, N. M. Provine, A. G. McNally, S. Li, F. R. Frankel, and D. H. Barouch. 2013. An Attenuated Listeria monocytogenes Vector Primes More Potent Simian Immunodeficiency Virus-Specific Mucosal Immunity than DNA Vaccines in Mice. J Virol 87: 4751–4755.
Penaloza-Macmaster, P., N. M. Provine, J. Ra, E. N. Borducchi, A. McNally, N. L. Simmons, M. J. Iampietro, and D. H. Barouch. 2013. Alternative serotype adenovirus vaccine vectors elicit memory T cells with enhanced anamnestic capacity compared to Ad5 vectors. J Virol 87: 1373–1384.
Provine, N. M., V. Cortez, V. Chohan, and J. Overbaugh. 2012. The neutralization sensitivity of viruses representing human immunodeficiency virus type 1 variants of diverse subtypes from early in infection is dependent on producer cell, as well as characteristics of the specific antibody and envelope variant. Virology 427: 25–33.
Provine, N. M.*, W. B. Puryear*, X. Wu, J. Overbaugh, and N. L. Haigwood. 2009. The infectious molecular clone and pseudotyped virus models of human immunodeficiency virus type 1 exhibit significant differences in virion composition with only moderate differences in infectivity and inhibition sensitivity. J Virol 83: 9002–9007. *Contributed equally