BRAF V600 mutation profiling in primary skin nodular melanoma in Indonesia: an analysis using high resolution pyrosequencing.

Rinonce HT., Aji RPM., Hayati N., Pudjohartono MF., Kameswari B., Anwar SL., Irianiwati .

ObjectiveWe aimed to investigate the prevalence and type of BRAF V600 mutations and the associations with clinicopathological variables in primary skin nodular melanoma cases in Yogyakarta and Central Java, Indonesia using pyrosequencing.ResultsBRAF V600 mutations of the V600E type were found in 21 (53.85%) specimens. The variant allele frequencies (VAFs) ranged from 5.07 to 94.70%, averaging 29.05%. However, most cases had low VAFs, with 13 (61.9%) specimens below 20% and 4 (19.05%) below 10%.

DOI

10.1186/s13104-020-05000-w

Type

Journal article

Publication Date

2020-03-01T00:00:00+00:00

Volume

13

Addresses

Department of Anatomical Pathology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Sleman, Yogyakarta, 55281, Indonesia. hanggoro_rinonce@ugm.ac.id.

Keywords

Humans, Melanoma, Skin Neoplasms, Proto-Oncogene Proteins B-raf, Cross-Sectional Studies, Adult, Aged, Aged, 80 and over, Middle Aged, Indonesia, Female, Male, Young Adult, High-Throughput Nucleotide Sequencing

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