Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

Original publication

DOI

10.1371/journal.pone.0184496

Type

Journal article

Journal

PloS one

Publication Date

11/10/2017

Volume

12

Pages

e0184496 - e0184496

Addresses

Harvard Medical School, Boston, Massachusetts, United States of America.

Keywords

CD8-Positive T-Lymphocytes, Humans, HIV Infections, Antiviral Agents, Lymphocyte Depletion, Endocytosis, Ribosome Inactivating Proteins, Type 1, Protein Multimerization